noti cloning sites Search Results


a plasmid construct containing a mutated, synthetic s . pseudintermedius spex  (GenScript corporation)
90
GenScript corporation a plasmid construct containing a mutated, synthetic s . pseudintermedius spex
a. SpEX of S . <t>pseudintermedius</t> harbors an N-terminal signal peptide (green arrow) from position 1–35, OB domain (orange arrow) in residues 43–126 and C-terminal β grasp domain in residues 150–234 (orange arrow). b. The 3D model of S . pseudintermedius SpEX and S . aureus SSL11 shows the N and C terminal domains fold into a five-stranded beta-barrel structure consisting of β1- β5 and β7, β6, β12, β9 and β10. A central helix is orientated diagonally in the center of the field from the top left to the bottom right leaving the N-terminal domain on the left and C-terminal domain on the right. A conserved sialated glycan binding domain forming V-shape depression is highlighted in purple in SpEX and SSL11 3D models.
A Plasmid Construct Containing A Mutated, Synthetic S . Pseudintermedius Spex, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/noti+cloning+sites/a+plasmid+construct+containing+a+mutated++synthetic+s++pseudintermedius+spex++designed+as+described+below++with+bamhi+noti+cloning+sites/pmc06663030-126-9-22
Average 90 stars, based on 1 article reviews
a plasmid construct containing a mutated, synthetic s . pseudintermedius spex - by Bioz Stars, 2026-09
90/100 stars
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noti cloning sites in pgex4t3  (Danaher Inc)
86
Danaher Inc noti cloning sites in pgex4t3
a. SpEX of S . <t>pseudintermedius</t> harbors an N-terminal signal peptide (green arrow) from position 1–35, OB domain (orange arrow) in residues 43–126 and C-terminal β grasp domain in residues 150–234 (orange arrow). b. The 3D model of S . pseudintermedius SpEX and S . aureus SSL11 shows the N and C terminal domains fold into a five-stranded beta-barrel structure consisting of β1- β5 and β7, β6, β12, β9 and β10. A central helix is orientated diagonally in the center of the field from the top left to the bottom right leaving the N-terminal domain on the left and C-terminal domain on the right. A conserved sialated glycan binding domain forming V-shape depression is highlighted in purple in SpEX and SSL11 3D models.
Noti Cloning Sites In Pgex4t3, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/noti+cloning+sites/pm12244126-87-11-16
Average 86 stars, based on 1 article reviews
noti cloning sites in pgex4t3 - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

Image Search Results


a. SpEX of S . pseudintermedius harbors an N-terminal signal peptide (green arrow) from position 1–35, OB domain (orange arrow) in residues 43–126 and C-terminal β grasp domain in residues 150–234 (orange arrow). b. The 3D model of S . pseudintermedius SpEX and S . aureus SSL11 shows the N and C terminal domains fold into a five-stranded beta-barrel structure consisting of β1- β5 and β7, β6, β12, β9 and β10. A central helix is orientated diagonally in the center of the field from the top left to the bottom right leaving the N-terminal domain on the left and C-terminal domain on the right. A conserved sialated glycan binding domain forming V-shape depression is highlighted in purple in SpEX and SSL11 3D models.

Journal: PLoS ONE

Article Title: Identification, cloning and characterization of SpEX exotoxin produced by Staphylococcus pseudintermedius

doi: 10.1371/journal.pone.0220301

Figure Lengend Snippet: a. SpEX of S . pseudintermedius harbors an N-terminal signal peptide (green arrow) from position 1–35, OB domain (orange arrow) in residues 43–126 and C-terminal β grasp domain in residues 150–234 (orange arrow). b. The 3D model of S . pseudintermedius SpEX and S . aureus SSL11 shows the N and C terminal domains fold into a five-stranded beta-barrel structure consisting of β1- β5 and β7, β6, β12, β9 and β10. A central helix is orientated diagonally in the center of the field from the top left to the bottom right leaving the N-terminal domain on the left and C-terminal domain on the right. A conserved sialated glycan binding domain forming V-shape depression is highlighted in purple in SpEX and SSL11 3D models.

Article Snippet: A plasmid construct containing a mutated, synthetic S . pseudintermedius spEX (designed as described below) with BamHI/NotI cloning sites, was obtained commercially (Genscript Piscataway, NJ USA) ( ).

Techniques: Glycoproteomics, Binding Assay

Recombinant SpEX at a concentration of 0.2 μg /ml significantly inhibited the migration of PMNs induced by fetal bovine serum compared to SpEX-M at the same concentration with P < 0.0001****. The chemotaxis inhibition by culture supernatant of S . pseudintermedius 06–3228 was significantly higher than SpEX P = 0.0035**. The values represent averages from three independent experiments. (*P < 0.05 was considered significant). Data are plotted on the Y axis X 1000.

Journal: PLoS ONE

Article Title: Identification, cloning and characterization of SpEX exotoxin produced by Staphylococcus pseudintermedius

doi: 10.1371/journal.pone.0220301

Figure Lengend Snippet: Recombinant SpEX at a concentration of 0.2 μg /ml significantly inhibited the migration of PMNs induced by fetal bovine serum compared to SpEX-M at the same concentration with P < 0.0001****. The chemotaxis inhibition by culture supernatant of S . pseudintermedius 06–3228 was significantly higher than SpEX P = 0.0035**. The values represent averages from three independent experiments. (*P < 0.05 was considered significant). Data are plotted on the Y axis X 1000.

Article Snippet: A plasmid construct containing a mutated, synthetic S . pseudintermedius spEX (designed as described below) with BamHI/NotI cloning sites, was obtained commercially (Genscript Piscataway, NJ USA) ( ).

Techniques: Recombinant, Concentration Assay, Migration, Chemotaxis Assay, Inhibition

Antibodies against S . pseudintermedius native SpEX and SpEX-M were measured using an indirect ELISA. Recombinant S . pseudintermedius SpEX and SpEX-M proteins were coated on ELISA plates, then incubated with two-fold serially diluted serum from dogs vaccinated with the same proteins. High reactivity with SpEX and SpEX-M occurred with sera collected two weeks after the third injections of SpEX-M (P <0.0001) compared to pre-injection sera. The values represent averages from three independent experiments.

Journal: PLoS ONE

Article Title: Identification, cloning and characterization of SpEX exotoxin produced by Staphylococcus pseudintermedius

doi: 10.1371/journal.pone.0220301

Figure Lengend Snippet: Antibodies against S . pseudintermedius native SpEX and SpEX-M were measured using an indirect ELISA. Recombinant S . pseudintermedius SpEX and SpEX-M proteins were coated on ELISA plates, then incubated with two-fold serially diluted serum from dogs vaccinated with the same proteins. High reactivity with SpEX and SpEX-M occurred with sera collected two weeks after the third injections of SpEX-M (P <0.0001) compared to pre-injection sera. The values represent averages from three independent experiments.

Article Snippet: A plasmid construct containing a mutated, synthetic S . pseudintermedius spEX (designed as described below) with BamHI/NotI cloning sites, was obtained commercially (Genscript Piscataway, NJ USA) ( ).

Techniques: Indirect ELISA, Recombinant, Enzyme-linked Immunosorbent Assay, Incubation, Injection

Bacteria, plasmids and competent cells used in this study.

Journal: PLoS ONE

Article Title: Identification, cloning and characterization of SpEX exotoxin produced by Staphylococcus pseudintermedius

doi: 10.1371/journal.pone.0220301

Figure Lengend Snippet: Bacteria, plasmids and competent cells used in this study.

Article Snippet: A plasmid construct containing a mutated, synthetic S . pseudintermedius spEX (designed as described below) with BamHI/NotI cloning sites, was obtained commercially (Genscript Piscataway, NJ USA) ( ).

Techniques: Bacteria, Construct, Cloning, Plasmid Preparation, Expressing, Recombinant, Control

Primers used to amplify recombinant wild- type and attenuated spEX from Staphylococcus pseudintermedius and pUC19- spEX- M plasmid. NotI and BamHI restriction sites are bold and underlined, nucleotide sequences belonging to SpEX and SpEX-M are show in italics, the primer binding sites are in brackets and sequences outside SpEX and SpEX-M (GCATGA) were used to flank NotI and BamHI recognition sites.

Journal: PLoS ONE

Article Title: Identification, cloning and characterization of SpEX exotoxin produced by Staphylococcus pseudintermedius

doi: 10.1371/journal.pone.0220301

Figure Lengend Snippet: Primers used to amplify recombinant wild- type and attenuated spEX from Staphylococcus pseudintermedius and pUC19- spEX- M plasmid. NotI and BamHI restriction sites are bold and underlined, nucleotide sequences belonging to SpEX and SpEX-M are show in italics, the primer binding sites are in brackets and sequences outside SpEX and SpEX-M (GCATGA) were used to flank NotI and BamHI recognition sites.

Article Snippet: A plasmid construct containing a mutated, synthetic S . pseudintermedius spEX (designed as described below) with BamHI/NotI cloning sites, was obtained commercially (Genscript Piscataway, NJ USA) ( ).

Techniques: Recombinant, Plasmid Preparation, Binding Assay, Sequencing